Order CGI-58 Antibody

Product Details

CGI-58 is a membrane protein that is essential for plant growth, development and lipid metabolism. We have shown previously that expression of mouse Order CGI-58 antibody  cells increases phosphatidylglycerol (PG) and decreases other major phospholipids including PC, PE, phosphatidylinositol, and phosphatidylserine. Interestingly, a mutant of plant CGI-58 lacking the putative catalytic seryl residue showed a similar effect on PG levels but not in other phospholipids. These observations led us to test the hypothesis that CGI-58 has a general lipid metabolic function and that it interacts with PXA1.

CGI-58 Antibody: Applications and Research Benefits

We have used the yeast two-hybrid system pGADT7-AD (Clontech) and the nuclear relocalization assay vector pRTL2/NLS-RFP (Dhanoa et al., 2010) to construct a yeast clone that expresses GFP-tagged plant CGI-58. This clone was subcloned into the pMDC43 binary vector for two-hybrid interaction with a PXA1 cDNA fragment.

Coimmunoprecipitation of the full-length recombinant proteins in vitro confirmed a physical interaction and mutagenesis studies indicated that the pore-forming domain of PXA1 is a key interacting region of CGI-58. Moreover, in a cgi-58 pxa1 double mutant, plants displayed reductions in jasmonate biosynthesis, auxin signaling, and lipid metabolism consistent with decreased PXA1 activity. We have also provided evidence that recombinant plant CGI-58, but not mouse CGI-58, exhibits LPAAT activity and is able to hydrolyze fatty acid substrates containing an HxxxxD motif. Moreover, a chimera of plsC containing the first 40 amino acids of mouse CGI-58 and an HxxxxD mutation of plant CGI-58 shows copper resistance in BL21(DE3) and a decrease in PG accumulation compared to a control.…

Guide to Tracking Devices With Fingerprinting API

Guide to tracking devices with fingerprinting API

Guide to tracking devices with fingerprinting API is a method used to establish a consistent unique identifier for a device or user over time. It is a form of cross-device tracking that can be leveraged to detect spoofing, emulators, bots and other malicious behavior, such as account sharing across multiple devices.

While the technology offers great promise for fraud prevention, there are privacy concerns that must be considered. Without greater transparency, users may not understand how or why their personal information is being collected and the purposes for which it will be used, leading to a lack of trust and questions about data ownership and control. Users can take steps to limit the effectiveness of fingerprinting by using privacy-enhancing browser extensions, disabling cookies or other tracking features, and by adjusting their device settings (e.g., by configuring JavaScript to block third-party cookies or by regularly clearing their browsing data).

Getting Started with IPQualityScore APIs: Easy Integration Steps

Fingerprinting also raises legal concerns, as it involves the collection of personal information, such as device information, without explicit consent. The California Consumer Privacy Act and Brazil’s LGPD (Lei Geral de Protecço da Dados) require that businesses clearly disclose their use of personal information to consumers and offer an opt-out mechanism. In addition, the GDPR requires that companies obtain explicit consent from users to process sensitive personal data (e.g., location, MAC address), including device information. To help address these concerns, the Fraud Protection service uses a combination of methods to assess risk, including device fingerprinting. This information is then integrated into the risk assessment API call to Microsoft Entra.…